CPC C12Q 1/6811 (2013.01) [C12Q 1/6816 (2013.01); C12Q 1/6851 (2013.01); C12Q 1/6853 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6876 (2013.01); C12Q 1/6888 (2013.01); C12Q 1/701 (2013.01); C12Q 2600/118 (2013.01); C12Q 2600/166 (2013.01)] | 17 Claims |
1. A composition comprising first, second, third, and fourth amplification oligomers, wherein:
the first amplification oligomer and second amplification oligomer are configured to produce an HPIV-3 amplicon of at least about 50 nucleotides in length comprising at least one Human Parainfluenza Virus 3 (HPIV-3) position located in the range of positions 1350-1360, wherein the first oligomer comprises a target-hybridizing region consisting of the sequence of SEQ ID NO: 25 and the second oligomer comprises a target-hybridizing region consisting of the sequence of SEQ ID NO: 36;
the third amplification oligomer and fourth amplification oligomer are configured to produce an HPIV-4 amplicon, wherein the third oligomer comprises a target-hybridizing region consisting of the sequence of SEQ ID NO: 78 and the fourth oligomer comprises a target-hybridizing region consisting of the sequence of SEQ ID NO: 56;
the composition further comprises a fifth oligomer configured to hybridize to the HPIV-3 amplicon;
the composition further comprises a sixth oligomer configured to hybridize to the HPIV-4 amplicon, wherein the sixth oligomer is a detection probe; and
the sixth oligomer comprises a non-nucleotide detectable label.
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