	US 12,116,590 B2
	Method for mass-producing plant exosomes
Yong-Woo Cho, Seongnam (KR); Ji-Suk Choi, Gunpo (KR); Young-Chan Choi, Chuncheon (KR); and Min-Kang Kim, Namyangju (KR)
Assigned to Industry-University Cooperation Foundation Hanyang University ERICA Campus, Ansan (KR); and EXOSTEMTECH CO., LTD., Gyeonggi-do (KR)
Appl. No. 17/623,857
Filed by Industry-University Cooperation Foundation Hanyang University ERICA Campus, Ansan (KR); and EXOSTEMTECH CO., LTD., Ansan (KR)
PCT Filed Apr. 10, 2020, PCT No. PCT/KR2020/004906 § 371(c)(1), (2) Date Dec. 29, 2021, PCT Pub. No. WO2021/002571, PCT Pub. Date Jan. 7, 2021.
Claims priority of application No. 10-2019-0079490 (KR), filed on Jul. 2, 2019.
Prior Publication US 2022/0364051 A1, Nov. 17, 2022
Int. Cl. C12N 15/82 (2006.01); B01D 61/14 (2006.01); B01D 61/16 (2006.01); C12N 5/04 (2006.01)

CPC C12N 5/04 (2013.01) [B01D 61/145 (2013.01); B01D 61/16 (2013.01); B01D 2311/2676 (2013.01)]

7 Claims

1. A method for preparing a high-purity plant exosome extract, the method comprising:

preparing a plant extract from a raw plant material;

performing centrifugation of the plant extract at 10,000 to 50,000×g, and obtaining a supernatant;

performing ultracentrifugation at 100,000 to 600,000×g using the supernatant, and obtaining a pellet;

suspending the pellet to obtain a suspension;

performing tangential-flow filtration (TFF) using the suspension; and

obtaining the high-purity plant exosome extract, wherein the high-purity plant exosome extract comprises plant exosomes at a concentration of 10⁷to 10¹²particles per 1 ml of the high purity plant exosome extract, and wherein the high purity plant exosome extract lacks vacuoles.