CPC C12N 15/8213 (2013.01) [A01H 1/08 (2013.01); C12N 15/8217 (2013.01)] | 17 Claims |
1. A synthetic transcription factor, or a nucleotide sequence encoding the same, comprising at least one recognition domain and at least one activation domain, wherein the synthetic transcription factor is configured to modulate the expression of an endogenous morphogenic gene in a cellular system, wherein the modulation is a targeted activation, and wherein:
wherein the morphogenic gene comprises a nucleotide sequence selected from the group consisting of:
(i) a nucleotide sequence set forth in any one of SEQ ID NOs: 199, 207, 217, 218, or 219;
(ii) a nucleotide sequence having the coding sequences of the nucleotide sequence set forth in SEQ ID NOs: 199, 207, 217, 218, or 219;
(iii) the nucleotide sequence complementary to the nucleotide sequence of (i) or (ii);
(iv) a nucleotide sequence having at least 95% identity to the nucleotide sequence of (i), (ii) or (iii); and
(v) a nucleotide sequence encoding a protein comprising the amino acid sequence set forth in any one of SEQ ID NOs: 238 to 240;
and
wherein the synthetic transcription factor comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 151 and SEQ ID NO: 218, and combinations thereof, and/or the at least one recognition domain comprises a sequence set forth in any one of SEQ ID NOs: 13 to 51 or SEQ ID NOs: 52 to 94, and
wherein the synthetic transcription factor and/or the at least one recognition domain binds to a regulation region having the sequence set forth in SEQ ID NOs: 95, 109 to 147 or SEQ ID NOs: 96, 148 to 190.
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