US 12,116,579 B2
Compositions and methods related to fungal hypoxia responsive morphology factor a (hrmA) and biofilm architecture factor (baf) proteins
Robert A. Cramer, Jr., Hanover, NH (US); and Caitlin Kowalski, Hanover, NH (US)
Assigned to THE TRUSTEES OF DARTMOUTH COLLEGE, Hanover, NH (US)
Appl. No. 17/642,592
Filed by THE TRUSTEES OF DARTMOUTH COLLEGE, Hanover, NH (US)
PCT Filed Sep. 11, 2020, PCT No. PCT/US2020/050352
§ 371(c)(1), (2) Date Mar. 11, 2022,
PCT Pub. No. WO2021/050840, PCT Pub. Date Mar. 18, 2021.
Claims priority of provisional application 62/899,660, filed on Sep. 12, 2019.
Claims priority of provisional application 63/006,930, filed on Apr. 8, 2020.
Prior Publication US 2023/0135464 A1, May 4, 2023
Int. Cl. C12N 15/80 (2006.01); C07K 14/38 (2006.01); C12N 15/52 (2006.01)
CPC C12N 15/80 (2013.01) [C07K 14/38 (2013.01); C12N 15/52 (2013.01)] 7 Claims
 
1. A filamentous fungal host cell, comprising an exogenous nucleotide sequence encoding an Aspergillus fumigatus biofilm architecture factor (baf) protein, wherein the baf protein comprises (a) bafA, or a homolog or ortholog thereof having bafA activity, (b) bafB, or a homolog or ortholog thereof having bafB activity, and (c) bafC, or a homolog or ortholog thereof having bafC activity, the bafA protein or a homolog or ortholog thereof having bafA activity comprising at least 90% identity to the amino acid sequence of SEQ ID NO: 13; the bafB protein or a homolog or ortholog thereof having bafB activity comprising at least 90% identity to the amino acid sequence of SEQ ID NO: 14; and the bafC protein or a homolog or ortholog thereof having bafC activity comprising at least 90% identity to the amino acid sequence of SEQ ID NO: 15.