US 12,116,572 B2
Target binding moiety compositions and methods of use
Xi Chen, Newton, MA (US); and Ely Porter, Medford, MA (US)
Assigned to Guangzhou Chengyuan Bioimmunology Technology Co., Ltd., Guangzhou (CN)
Filed by Guangzhou Chengyuan Bioimmunology Technology Co., Ltd., Guangdong (CN)
Filed on Dec. 15, 2022, as Appl. No. 18/066,463.
Application 18/066,463 is a continuation of application No. 17/031,260, filed on Sep. 24, 2020, granted, now 11,560,560.
Application 17/031,260 is a continuation of application No. PCT/US2019/023820, filed on Mar. 25, 2019.
Claims priority of provisional application 62/686,858, filed on Jun. 19, 2018.
Claims priority of provisional application 62/648,218, filed on Mar. 26, 2018.
Prior Publication US 2023/0279384 A1, Sep. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); G01N 33/68 (2006.01)
CPC C12N 15/1065 (2013.01) [G01N 33/6854 (2013.01); G01N 2458/10 (2013.01)] 20 Claims
 
1. A method for manufacturing a polynucleotide barcoded target binding moiety, the method comprising:
(a) translating an RNA sequence of an RNA molecule, wherein the RNA sequence encodes a peptide sequence, wherein the RNA molecule is linked to a peptide acceptor at a 3′ end of the RNA molecule; and
(b) linking the peptide acceptor to an amino acid residue of a translated peptide comprising the peptide sequence, thereby forming a nucleic acid-peptide fusion molecule, wherein the nucleic acid-peptide fusion molecule comprises a polynucleotide barcoded target binding moiety comprising:
(i) a first peptide sequence comprising a first binding region, and
(ii) a second peptide sequence comprising a second binding region;
wherein the first binding region and the second binding region are
(i) separated by a spacer, and
(ii) spaced at a distance such that the first peptide sequence and the second peptide sequence simultaneously bind to a single molecule comprising an antigen binding domain of an antibody.