US 12,116,559 B2
Method of manufacturing a recombinant polypeptide
Piia Kristiina Valonen, Kuopio (FI); Hanna P. Lesch, Kuopio (FI); Eva Kristiina Rasanen, Hiltulanlahti (FI); Tarja Hannele Tuunanen, Kuopio (FI); Minna Kristiina Karhinen, Kuopio (FI); and Seppo Ylä-Herttuala, Kuopio (FI)
Assigned to Trizell Ltd., West Drayton (GB)
Filed by Trizell Ltd., West Drayton (GB)
Filed on Dec. 22, 2021, as Appl. No. 17/559,366.
Application 17/559,366 is a division of application No. 15/579,208, granted, now 11,261,416, previously published as PCT/US2017/025681, filed on Apr. 3, 2017.
Claims priority of provisional application 62/322,651, filed on Apr. 14, 2016.
Prior Publication US 2022/0364036 A1, Nov. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/00 (2006.01); C07K 14/005 (2006.01); C12M 1/02 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01); C12N 15/86 (2006.01); C12P 21/02 (2006.01)
CPC C12M 29/18 (2013.01) [C07K 14/005 (2013.01); C12M 1/02 (2013.01); C12M 25/18 (2013.01); C12M 29/10 (2013.01); C12M 41/26 (2013.01); C12M 41/46 (2013.01); C12N 15/86 (2013.01); C12P 21/02 (2013.01); C12N 2740/15051 (2013.01)] 6 Claims
 
1. A method of manufacturing a recombinant polypeptide comprising:
(a) culturing producer cells comprising a nucleic acid sequence encoding the recombinant polypeptide in adherent mode in an adherent-cell culture bioreactor, wherein the adherent-cell culture bioreactor comprises:
(i) a substrate for adherent cell growth; and
(ii) a feed-in pump and a feed-out pump, wherein the feed-in pump and the feed-out pump are configured to provide a constant-rate output of cell culture media of less than about 50 mL of media per 1,800 cells per day; and
(b) providing a constant-rate output of cell culture media at a pump output volume sufficient to maintain a parameter in the cell culture media selected from the group consisting of: concentration of lactate in said medium of not more than about 1.58 grams/liter; and concentration of glucose in said medium of between about 0.5 and 1.0 grams/liter,
thereby manufacturing the recombinant polypeptide.