US 12,115,530 B2
On-board reagent storage in a fluid processing device
Christopher Kilcoin, La Jolla, CA (US); and Eugene Tu, Portland, OR (US)
Assigned to Lost Arrow Bio, La Jolla, CA (US)
Filed by Lost Arrow Bio, La Jolla, CA (US)
Filed on Mar. 18, 2022, as Appl. No. 17/698,504.
Claims priority of provisional application 63/163,535, filed on Mar. 19, 2021.
Prior Publication US 2022/0297123 A1, Sep. 22, 2022
Int. Cl. B01L 3/00 (2006.01)
CPC B01L 3/502715 (2013.01) [B01L 2200/025 (2013.01); B01L 2200/027 (2013.01); B01L 2200/04 (2013.01); B01L 2200/14 (2013.01); B01L 2200/16 (2013.01); B01L 2300/042 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/14 (2013.01); B01L 2400/0633 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A fluid processing device comprising:
two or more fluid channels;
one or more processing stations, wherein the two or more fluid channels pass through each processing station, and wherein each processing station comprises:
a process chamber associated with each of the two or more fluid channels;
a reagent pump assembly associated with each of the two or more fluid channels;
a reagent input well associated with each of the two or more fluid channels; and
a reagent channel associated with each reagent input well connecting each reagent input well to the process chamber of the associated fluid channel, wherein the reagent pump assemblies of each processing station are configured to be operable in unison to simultaneously move a reagent from each reagent input well of the associated fluid channel through the associated reagent channel and into each process chamber of the associated fluid channel;
a reagent pack associated with at least one of the one or more processing stations, wherein the reagent pack includes at least one reagent blister associated with each reagent input well of the associated processing station;
a barb actuator rod associated with the at least one processing station and including a barb associated with each reagent blister, wherein each barb actuator rod is configured to be movable between a first position in which each barb of the barb actuator rod punctures the at least one reagent blister associated with the barb and a second position in which each barb of the barb actuator rod does not contact the associated reagent blister, and wherein each reagent blister is positioned with respect to the associated reagent input well so that when the reagent blister is punctured by the associated barb, liquid content of the reagent blister is released into the associated reagent input well;
a reagent valve associated with each reagent pump assembly and movable between a first position fluidly connecting the reagent pump assembly to the associated reagent input well and a second position fluidly connecting the reagent pump assembly to the associated process chamber; and
a reagent valve rod associated with each of the one or more processing stations and coupled to every reagent valve of the reagent pump assemblies of the processing station so that movement of the reagent valve rod effects simultaneous movement of the reagent valves between their respective first and second positions, and wherein the barb actuator rod is coupled to the reagent valve rod, so that the barb actuator rod moves between its respective first and second positions as the reagent valve rod moves between its respective first and second positions.