CPC C12Q 1/6874 (2013.01) [A61K 47/00 (2013.01); C07K 16/2851 (2013.01); C07K 16/44 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6876 (2013.01); C07K 2317/73 (2013.01)] | 47 Claims |
1. A method for identifying a nucleobase of a non-labeled reversible terminator (NLRT) deoxyribonucleotide incorporated at a 3′ terminus of a primer extension product, wherein the NLRT deoxyribonucleotide comprises a nucleobase, a sugar moiety, and a cleavable blocking group, said method comprising
(a) combining (i) the primer extension product including the incorporated NLRT deoxyribonucleotide and (ii) a first affinity reagent that binds to the incorporated NLRT deoxyribonucleotide, wherein the first affinity reagent binds to the nucleobase, the cleavable blocking group, or both, and
(b) detecting binding of the first affinity reagent to the incorporated NLRT deoxyribonucleotide, wherein binding of the first affinity reagent identifies a nucleobase associated with the incorporated NLRT deoxyribonucleotide.
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