US RE50,636 E1
Accurate molecular barcoding
Eleen Shum, Redwood City, CA (US); and Glenn Fu, Dublin, CA (US)
Assigned to Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Oct. 27, 2022, as Appl. No. 18/050,449.
Application 18/050,449 is a reissue of application No. 15/581,914, filed on Apr. 28, 2017, granted, now 10,822,643, issued on Nov. 3, 2020.
Claims priority of provisional application 62/330,500, filed on May 2, 2016.
Int. Cl. C12Q 1/6837 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6837 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01)] 54 Claims
 
[ 25. A composition comprising:
at least 1000 unique oligonucleotide species, each unique oligonucleotide species comprising a barcode region and a uniform region, the barcode region comprising a molecule barcode comprising at least 7 nucleotides and having a constraint on G content,
wherein the uniform region is 3′ of the barcode region, the uniform region comprising a target-specific region 3′ of the barcode region, the target-specific region comprising at least 10 nucleotides complementary to a target nucleic acid,
wherein the unique oligonucleotide species comprise different nucleic acid sequences in their barcode regions,
wherein the constraint on G content comprises:
(a) less than 1% of the unique oligonucleotide species comprising the molecule barcode having a G content of 50% or more; and/or
(b) the molecule barcodes of all of the unique oligonucleotide species in the composition collectively have a G content of no more than 12.5%, and
wherein the barcode region further comprises a sample barcode comprising at least 3 nucleotides. ]