US 12,444,480 B2
Methods for genome assembly and haplotype phasing
Richard E. Green, Jr., Santa Cruz, CA (US); and Liana F. Lareau, San Francisco, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Feb. 8, 2024, as Appl. No. 18/436,754.
Application 18/436,754 is a continuation of application No. 17/066,868, filed on Oct. 9, 2020, granted, now 11,935,626.
Application 17/066,868 is a continuation of application No. 15/900,723, filed on Feb. 20, 2018, granted, now 10,825,553, issued on Nov. 3, 2020.
Application 15/900,723 is a continuation of application No. 15/167,880, filed on May 27, 2016, granted, now 9,910,955, issued on Mar. 6, 2018.
Application 15/167,880 is a continuation of application No. 14/170,339, filed on Jan. 31, 2014, granted, now 9,411,930, issued on Aug. 9, 2016.
Claims priority of provisional application 61/759,941, filed on Feb. 1, 2013.
Prior Publication US 2024/0395360 A1, Nov. 28, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6874 (2018.01); G16B 30/00 (2019.01); G16B 30/20 (2019.01)
CPC G16B 30/20 (2019.02) [C12Q 1/6874 (2013.01); G16B 30/00 (2019.02)] 18 Claims
OG exemplary drawing
 
1. A method for haplotype phasing, the method comprising:
(a) crosslinking deoxyribonucleic acid (DNA) within chromosomes to form crosslinked DNA;
(b) fragmenting the crosslinked DNA to form fragmented crosslinked DNA;
(c) ligating regions of the fragmented crosslinked DNA to create a plurality of ligation products, wherein at least two of the regions comprise an intra-chromosomal pair;
(d) sequencing the plurality of ligation products or a derivative thereof to generate sequencing reads; and
(e) using the sequencing reads to determine haplotype phasing.