US 12,444,477 B2
Antibody selection apparatus and methods
Vikas K. Sharma, Millbrae, CA (US); Trevor E. Swartz, San Carlos, CA (US); and Thomas W. Patapoff, San Carlos, CA (US)
Assigned to GENENTECH, INC., South San Francisco, CA (US)
Filed by GENENTECH, INC., South San Francisco, CA (US)
Filed on Nov. 12, 2020, as Appl. No. 17/095,970.
Application 17/095,970 is a continuation of application No. 15/166,939, filed on May 27, 2016, granted, now 10,861,584.
Application 15/166,939 is a continuation of application No. PCT/US2014/067580, filed on Nov. 26, 2014.
Claims priority of provisional application 61/910,200, filed on Nov. 29, 2013.
Prior Publication US 2021/0065843 A1, Mar. 4, 2021
Int. Cl. G16B 15/00 (2019.01); C07K 16/00 (2006.01); G01N 33/531 (2006.01); G16B 35/00 (2019.01); G16B 35/20 (2019.01); G16C 20/60 (2019.01)
CPC G16B 15/00 (2019.02) [C07K 16/00 (2013.01); G01N 33/531 (2013.01); G16B 35/00 (2019.02); G16B 35/20 (2019.02); G16C 20/60 (2019.02); C07K 2317/10 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A computer-implemented method comprising:
obtaining a clearance rate criterion for an antibody;
calculating a net charge using a light chain variable domain (VL) amino acid sequence and a heavy chain variable domain (VH) amino acid sequence of the antibody;
determining the net charge is within a specific net charge range;
calculating, using a molecular dynamics (MD) simulation, a hydrophobicity from one or more complementarity determining regions (CDRs) of the antibody;
determining the hydrophobicity is within a hydrophobicity range;
determining that the antibody has a predicted clearance rate that is below or equal to the clearance rate criterion;
determining that the antibody is suitable for inclusion in a pharmaceutical product; and
manufacturing the antibody and/or the pharmaceutical product comprising the antibody, wherein the manufactured antibody comprises: (i) the net charge being within the specific net charge range, (ii) the hydrophobicity being within the hydrophobicity range, and (iii) the predicted clearance rate being below or equal to the clearance rate criterion.