US 12,443,021 B2
Microscope for imaging a sample
Petr Strnad, Lausanne (CH); Andrea Boni, Prilly (CH); Prisca Liberali, Zürich (CH); and Gustavo Quintas Glasner De Medeiros, Lörrach (DE)
Assigned to VIVENTIS MICROSCOPY SÀRL, Lausanne (CH)
Appl. No. 18/262,128
Filed by VIVENTIS MICROSCOPY SÀRL, Lausanne (CH)
PCT Filed Jan. 21, 2022, PCT No. PCT/EP2022/051329
§ 371(c)(1), (2) Date Jul. 19, 2023,
PCT Pub. No. WO2022/157300, PCT Pub. Date Jul. 28, 2022.
Claims priority of application No. 00061/21 (CH), filed on Jan. 22, 2021.
Prior Publication US 2024/0094516 A1, Mar. 21, 2024
Int. Cl. G02B 21/00 (2006.01); G02B 21/18 (2006.01); G02B 21/36 (2006.01)
CPC G02B 21/0032 (2013.01) [G02B 21/0076 (2013.01); G02B 21/18 (2013.01); G02B 21/367 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A microscope comprising:
a sample holder configured to receive a sample and having a light transparent portion;
a first illumination objective arranged to eject a first illumination light beam along a first illumination path to illuminate the sample received by the sample holder through the light transparent portion of the sample holder such that light is ejected from the sample;
a second illumination objective arranged to eject a second illumination light beam along a second illumination path to illuminate the sample received by the sample holder through the light transparent portion of the sample holder such that light is ejected from the sample;
a first imaging objective arranged to receive detection light comprising at least a portion of the light ejected from the sample, wherein the detection light is propagated along a first detection path angled at about 90° to the first and second illumination paths and wherein the first imaging objective has a first imaging focal plane;
a second imaging objective arranged to receive detection light comprising at least a portion of the light ejected from the sample, wherein the detection light is propagated along a second detection path angled at about 90° to the first and second illumination paths and wherein the second imaging objective has a second imaging focal plane; and
a holder support arranged to support the sample holder, wherein, relative to a direction of gravity, the first and second illumination objectives are arranged such that the first and second illumination path are located below a bottom of the sample holder,
wherein the first and second illumination paths are not parallel, and
wherein the holder support has a drive system arranged to displace the sample holder along two perpendicular axes relative to the first and second imaging objectives.