	US 12,442,817 B2
	Means to detect whether acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus is virulent or non-virulent
Peter Bossier, De Pinte (BE); Vikash Kumar, Ghent (BE); and Suvra Roy, Ghent (BE)
Assigned to Universiteit Gent, Ghent (BE)
Appl. No. 17/641,402
Filed by Universiteit Gent, Ghent (BE)
PCT Filed Sep. 10, 2020, PCT No. PCT/EP2020/075292 § 371(c)(1), (2) Date Mar. 8, 2022, PCT Pub. No. WO2021/048258, PCT Pub. Date Mar. 18, 2021.
Claims priority of application No. 19197064 (EP), filed on Sep. 12, 2019.
Prior Publication US 2022/0326237 A1, Oct. 13, 2022
Int. Cl. G01N 33/569 (2006.01); G01N 33/573 (2006.01)

CPC G01N 33/56911 (2013.01) [G01N 33/573 (2013.01); G01N 2333/28 (2013.01); G01N 2333/916 (2013.01)]

7 Claims

1. A method of phenotyping Vibrio parahaemolyticus as non-virulent, the method comprising:

providing a sample comprising shrimp culture water;

utilizing antibodies or fragments thereof that bind to alkaline phosphatase Phox enzyme of V. parahaemolyticus to phenotype the V. parahaemolyticus as non-virulent; and

culturing the shrimp without disease-mitigation treatment if the V. parahaemolyticus are phenotyped as non-virulent.