	US 12,442,815 B1
	Viscoelastic hydrogel regulation of organoid patterning and vascularization
Changchun Zeng, Tallahassee, FL (US); Yan Li, Tallahassee, FL (US); and Xingchi Chen, Tallahassee, FL (US)
Assigned to The Florida State University Research Foundation, Inc., Tallahassee, FL (US)
Filed by The Florida State University Research Foundation, Inc., Tallahassee, FL (US)
Filed on Nov. 19, 2024, as Appl. No. 18/952,423.
Claims priority of provisional application 63/697,111, filed on Sep. 20, 2024.
Int. Cl. C12N 5/077 (2010.01); C12N 5/071 (2010.01); C12N 5/079 (2010.01); G01N 33/50 (2006.01)

CPC G01N 33/5082 (2013.01) [C12N 5/0618 (2013.01); C12N 5/0691 (2013.01); C12N 5/0697 (2013.01); G01N 33/5023 (2013.01); C12N 2513/00 (2013.01); C12N 2533/40 (2013.01); C12N 2537/10 (2013.01)]

13 Claims

1. A spinal cord model comprising:

a cell scaffold comprising methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat); and

a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold and expressing one or more ventral markers, dorsal markers, and/or interneuron markers.