US 12,442,769 B2
High-stability surface-enhanced raman scattering nanotag and making method therefor
Xiangwei Zhao, Nanjing (CN); and Ruihua Fei, Nanjing (CN)
Assigned to SOUTHEAST UNIVERSITY, Nanjing (CN)
Appl. No. 18/008,575
Filed by SOUTHEAST UNIVERSITY, Nanjing (CN)
PCT Filed Feb. 16, 2022, PCT No. PCT/CN2022/076392
§ 371(c)(1), (2) Date Dec. 6, 2022,
PCT Pub. No. WO2023/284296, PCT Pub. Date Jan. 19, 2023.
Claims priority of application No. 202110793566.4 (CN), filed on Jul. 14, 2021.
Prior Publication US 2025/0052684 A1, Feb. 13, 2025
Int. Cl. G01N 21/65 (2006.01)
CPC G01N 21/658 (2013.01) 9 Claims
OG exemplary drawing
 
1. A method for preparing a high-stability SERS nanotag, comprising the following steps:
(a) adding tetratetrachloroauric acid into ultrapure water, quickly adding a reducing agent with a mass to volume ratio of 1% to 2% after boiling, continuously carrying out boiling, stopping heating, and carrying out cooling to 24° C. to 37° C.;
(b) adding CTAC with a concentration of 0.01 mM to 1 mM into a substance obtained in the step (a), carrying out stirring, adding ascorbic acid, injecting AgNO3, and carrying out centrifugation after reaction and resuspending in CTAC with a concentration of 1 mM to 10 mM; and
(c) adding CTAC with a concentration of 20 mM to 100 mM into a substance obtained in the step (b), carrying out stirring, sequentially adding
a Raman dye, ascorbic acid and sodium hydroxide, injecting a mixture of tetratetrachloroauric acid and potassium iodide, and carrying out centrifugation after reaction and resuspending in CTAC with a concentration of 1 mM to 10 mM;
wherein the high-stability SERS nanotag comprises a gold core (1), wherein the gold core (1) is sequentially coated with a silver shell (2) and a gold shell (3), and a Raman dye (4) is arranged between the silver shell (2) and the gold shell (3).