US 12,442,768 B2
Photostimulation device and methods of using the same
William Benman, Philadelphia, PA (US); Lukasz Bugaj, Philadelphia, PA (US); Brian Chow, Philadelphia, PA (US); Saachi Datta, Atlanta, GA (US); David Gonzalez-Martinez, Philadelphia, PA (US); and Jose Avalos, Princeton, NJ (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US); and The Trustees of Princeton University, Princeton, NJ (US)
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US); and THE TRUSTEES OF PRINCETON UNIVERSITY, Princeton, NJ (US)
Filed on Sep. 14, 2023, as Appl. No. 18/467,469.
Claims priority of provisional application 63/375,641, filed on Sep. 14, 2022.
Prior Publication US 2024/0085332 A1, Mar. 14, 2024
Int. Cl. G01N 21/64 (2006.01)
CPC G01N 21/6486 (2013.01) [G01N 2201/0446 (2013.01); G01N 2201/0627 (2013.01); G01N 2201/064 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A stimulation and measurement device comprising:
a microwell plate comprising:
a top side;
a bottom side; and
at least one well extending from the top side towards the bottom side;
a first assembly removably positioned on the top side of the microwell plate, the first assembly comprising:
a first printed circuit board; and
at least one pair of stimulating light emitting diodes (LEDs), each of the at least one pair of stimulating LEDs arranged and disposed on the first printed circuit board to align with one of the at least one wells;
a second assembly removably positioned on the bottom side of the microwell plate, the second assembly comprising:
a second printed circuit board;
at least one photodiode, each of the at least one photodiodes arranged and disposed on the second printed circuit board to align with one of the at least one pairs of stimulating LEDs; and
at least one excitation LED paired with each of the at least one photodiodes; and
a microcontroller configured to control the at least one pair of stimulating LEDs, the at least one photodiode, and the at least one excitation LED.