US 12,442,751 B2
Flow cell
Johnathan Charles Sharpe, Hamilton (NZ); Rudolf Hulspas, Maynard, MA (US); and Kristopher Scott Buchanan, Fort Collins, CO (US)
Assigned to CYTONOME/ST, LLC, Lexington, MA (US)
Filed by CYTONOME/ST, LLC, Bedford, MA (US)
Filed on Aug. 7, 2019, as Appl. No. 16/534,759.
Application 16/534,759 is a continuation of application No. 14/030,946, filed on Sep. 18, 2013, granted, now 10,379,029.
Claims priority of provisional application 61/702,532, filed on Sep. 18, 2012.
Prior Publication US 2020/0072731 A1, Mar. 5, 2020
Int. Cl. G01N 15/1434 (2024.01); G01N 15/14 (2024.01); G01N 15/1404 (2024.01); G01N 15/149 (2024.01)
CPC G01N 15/1436 (2013.01) [G01N 15/1404 (2013.01); G01N 15/1459 (2013.01); G01N 15/149 (2024.01); Y10T 436/25375 (2015.01)] 16 Claims
OG exemplary drawing
 
1. A droplet sorting method comprising:
forming a hydrodynamically focused stream of particles by flowing a stream including particles through at least one hydrodynamic focusing region provided in an operatively sealed sort chamber;
introducing the hydrodynamically focused stream of particles through a jet-forming element into an enclosed region provided in the sort chamber;
applying excitation light from an excitation source to the hydrodynamically focused stream of particles downstream of the jet-forming element;
transforming the hydrodynamically focused stream of particles into a series of droplets; and
deflecting a droplet from the series of droplets in a droplet deflection region based on a charge applied to the droplet.