US 12,442,750 B2
Methods and systems for identifying a fluorochrome panel
Peter Mage, San Jose, CA (US); and Mirko Corselli, Encinitas, CA (US)
Assigned to BECTON, DICKINSON AND COMPANY, Franklin Lakes, NJ (US)
Filed by Becton, Dickinson and Company, Franklin Lakes, NJ (US)
Filed on Dec. 19, 2022, as Appl. No. 18/083,808.
Claims priority of provisional application 63/305,010, filed on Jan. 31, 2022.
Prior Publication US 2023/0243735 A1, Aug. 3, 2023
Int. Cl. G01N 15/1434 (2024.01); G01N 33/58 (2006.01); G01N 15/10 (2024.01)
CPC G01N 15/1434 (2013.01) [G01N 33/582 (2013.01); G01N 2015/1006 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of identifying a fluorochrome panel suitable for use in a flow cytometric protocol, the method comprising:
receiving:
an instrument identifier; and
a request for N fluorochrome identifiers, where N is an integer of at least 2;
selecting two or more subsets of N fluorochrome identifiers from a set of fluorochrome identifiers in a spectral matrix associated with the instrument identifier, wherein each fluorochrome identifier in the spectral matrix is associated with a spectral signature;
identifying the fluorochrome panel from the two or more subsets of N fluorochrome identifiers based on a numerical stability metric calculated for each of the two or more subsets of N fluorochrome identifiers using the spectral signatures associated with the two or more subsets of N fluorochrome identifiers.