US 12,442,748 B1
Stimulus response testing using automated flow cytometry protocols
Shervin Javadi, San Jose, CA (US)
Assigned to Shero Diagnostics, San Jose, CA (US)
Filed by Stratedigm, Inc., San Jose, CA (US)
Filed on Nov. 19, 2021, as Appl. No. 17/531,504.
Claims priority of provisional application 63/116,706, filed on Nov. 20, 2020.
Int. Cl. G01N 15/14 (2024.01); G01N 33/58 (2006.01)
CPC G01N 15/14 (2013.01) [G01N 33/582 (2013.01); G01N 2015/1402 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for automated testing of a response of a sample to a chemical stimulus, the method comprising:
measuring, using flow cytometry of a first portion of the sample, one or more fluorescence intensities associated with activation of the first portion as induced by a first concentration of the chemical stimulus;
determining, using a computer system, whether an activation response is greater than a preselected threshold, wherein the activation response is a function of the one or more measured fluorescence intensities; and
providing an operation configured to be automatically performed by the computer system only if the determination concludes that the activation response is greater than the preselected threshold, wherein the operation comprises:
quantifying, using flow cytometry of a second portion of the sample, one or more fluorescence intensities associated with activation of the second portion as induced by a second concentration of the chemical stimulus, wherein the second concentration is lower than the first concentration.