US 12,442,732 B2
Organoids and methods of making and using the same
David L. Kaplan, Concord, MA (US); and Rachael N. Parker, Medford, MA (US)
Assigned to Trustees of Tufts College, Medford, MA (US)
Filed by Trustees of Tufts College, Medford, MA (US)
Filed on Apr. 22, 2021, as Appl. No. 17/302,069.
Claims priority of provisional application 63/110,333, filed on Nov. 5, 2020.
Claims priority of provisional application 63/014,045, filed on Apr. 22, 2020.
Prior Publication US 2021/0333178 A1, Oct. 28, 2021
Int. Cl. G01N 1/30 (2006.01)
CPC G01N 1/30 (2013.01) [G01N 2001/305 (2013.01)] 20 Claims
 
1. An embedded organoid for improved histological sample preparation comprising:
an organoid embedded within a hydrogel matrix, the hydrogel matrix having a chemical composition consisting essentially of one or more silk-elastin-like polymers (SELPs), water, and an optional bulking agent, wherein the hydrogel matrix has undergone a temperature-induced shape change to encapsulate the organoid following placement of the organoid at a receiving area of the hydrogel matrix, and
wherein subjecting the embedded organoid to histological sample preparation involving sectioning the embedded organoid results in a histological sample with reduced organoid damage when compared to a comparison histological sample prepared by subjecting a non-embedded organoid to the histological sample preparation involving sectioning the non-embedded organoid.