US 12,442,048 B2
Assays for detecting multiple tick-borne pathogens
Adalberto A. Perez de Leon, Wake Forest, NC (US); Maria D. Esteve-Gasent, College Station, TX (US); Pamela J. Ferro, Bryan, TX (US); and Joseph J. Modarelli, Bluffton, TX (US)
Assigned to THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE, Washington, DC (US)
Filed by THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE, Washington, DC (US); and THE TEXAS A&M UNIVERSITY SYSTEM, College Station, TX (US)
Filed on Apr. 13, 2021, as Appl. No. 17/228,749.
Application 17/228,749 is a continuation of application No. 16/130,177, filed on Sep. 13, 2018, abandoned.
Claims priority of provisional application 62/563,780, filed on Sep. 27, 2017.
Prior Publication US 2021/0230673 A1, Jul. 29, 2021
Int. Cl. C12Q 1/68 (2018.01); C07H 21/04 (2006.01); C12Q 1/6806 (2018.01); C12Q 1/686 (2018.01); C12Q 1/689 (2018.01)
CPC C12Q 1/689 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 2600/112 (2013.01); C12Q 2600/16 (2013.01); C12Q 2600/166 (2013.01)] 8 Claims
 
1. A kit for detecting Borrelia spp., Rickettsia-like bacteria, and Babesia spp. in a sample comprising:
at least one primer set for detecting said Borrelia spp. comprising SEQ ID NOs: 1 and 2;
at least one primer set for detecting said Rickettsia-like bacteria comprising SEQ ID NOs: 21 and 22;
at least one primer set for detecting said Babesia spp. comprising SEQ ID NOs: 38 and 39,
at least one labeled probe comprising SEQ ID NO: 3 for detecting the presence of at least one Borrelia spp. amplicon, at least one labeled probe comprising SEQ ID NO: 23 for detecting the presence of a Rickettsia-like bacteria amplicon, and at least one labeled probe comprising SEQ ID NO: 40 for detecting the presence of a Babesia spp. amplicon, said probes being labeled with a fluorescent label and a quencher,
an amplification control DNA,
instructions for using said primer sets, said probes and said amplification control DNA,
optionally PCR reagents, and
optionally reagents for isolating DNA from a sample.