US 12,442,032 B2
Solid support for sample collection
Peter James Tatnell, Cardiff (GB); and Nina Moran, Bristol (GB)
Assigned to Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
Filed by Global Life Sciences Solutions Operations UK Ltd, Sheffield (GB)
Filed on Apr. 22, 2021, as Appl. No. 17/237,518.
Application 17/237,518 is a division of application No. 16/078,787, abandoned, previously published as PCT/EP2017/054636, filed on Feb. 28, 2017.
Claims priority of application No. 1603459 (GB), filed on Feb. 29, 2016.
Prior Publication US 2021/0238656 A1, Aug. 5, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); G01N 1/02 (2006.01); G01N 1/10 (2006.01)
CPC C12Q 1/6806 (2013.01) [G01N 1/02 (2013.01); G01N 1/10 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for collecting a biological sample comprising, applying a biological sample to a solid support, wherein said sample support comprises:
a dye selected from the group consisting of Alizarin Red, Methylene blue, Reactive blue 4, Alcian blue, and Indigo carmine;
Tris HCl;
Tris EDTA;
SDS; and
uric acid;
wherein when the dye is Alizarin Red, the Alizarin Red is used at a concentration of about 0.3 to about 3.9 mM,
wherein when the dye is Methylene blue, the Methylene blue is used at a concentration of about 0.3 to about 4.0 mM,
wherein when the dye is Reactive blue 4, the Reactive blue 4 is used at a concentration of about 0.31 to about 3.14 mM,
wherein when the dye is Alcian blue, the Alcian blue is used at a concentration of about 0.1 to about 5 mM,
wherein when the dye is Indigo carmine, the Indigo carmine is used at a concentration of about 0.2 to about 7.5 mM,
wherein the solid support is configured to collect a biological sample,
wherein the solid support comprises a cellulose-based paper that is coated or impregnated with the Tris HCl, the Tris EDTA, the SDS, and the uric acid, and
wherein the dye is resistant to one or more of colour changes or colour fading, when exposed to one or more of ethylene oxide or ultra violet radiation.