	US 12,442,027 B2
	Method of reducing aromatic nitro compounds
Serena Bisagni, Cambridge (GB); Amin Bornadel, Cambridge (GB); Beatriz Dominguez, Cambridge (GB); Jacques Lepaih, Cambridge (GB); Steven M. Mennen, Thousand Oaks, CA (US); Ahir Pushpanath, Cambridge (GB); Iustina Slabu, Cambridge (GB); Jason Tedrow, Thousand Oaks, CA (US); and Simon J. Hedley, Thousand Oaks, CA (US)
Assigned to Amgen Inc., Thousand Oaks, CA (US)
Appl. No. 17/309,727
Filed by Amgen Inc., Thousand Oaks, CA (US)
PCT Filed Dec. 12, 2019, PCT No. PCT/US2019/066026 § 371(c)(1), (2) Date Jun. 16, 2021, PCT Pub. No. WO2020/131574, PCT Pub. Date Jun. 25, 2020.
Claims priority of provisional application 62/781,124, filed on Dec. 18, 2018.
Prior Publication US 2022/0042055 A1, Feb. 10, 2022
Int. Cl. C12P 17/12 (2006.01); C07D 213/73 (2006.01); C07D 295/205 (2006.01)

CPC C12P 17/12 (2013.01) [C07D 213/73 (2013.01); C07D 295/205 (2013.01)]

15 Claims

1. A method of reducing an aromatic nitro compound comprising the step of:

(i) contacting an aromatic nitro compound with a catalyst at ambient temperature and pressure,

wherein, the catalyst comprises:

(a) a disproportionation agent; and

(b) a nitroreductase comprising the amino acid sequence of SEQ ID NO:1 [NR-4], SEQ ID NO:2 [NR-14], SEQ ID NO:3 [NR-17] or SEQ ID NO: 6 [NR-24], and

wherein the aromatic nitro compound is 2-methyl-5-nitropyridine and the conversion of the aromatic nitro compound is greater than 90%.