US 12,442,026 B2
Production of fatty acyl-CoA in yeast using a fatty acid feedstock
Alexander Hutagalung, San Diego, CA (US); and Jose Miguel Laplaza, San Diego, CA (US)
Assigned to Pyrone Systems, Inc., San Diego, CA (US)
Filed by Pyrone Systems, Inc., San Diego, CA (US)
Filed on Jan. 24, 2024, as Appl. No. 18/421,170.
Application 18/421,170 is a continuation of application No. 17/449,847, filed on Oct. 4, 2021, granted, now 11,884,948.
Application 17/449,847 is a continuation of application No. 16/783,122, filed on Feb. 5, 2020, granted, now 11,136,605, issued on Oct. 5, 2021.
Application 16/783,122 is a continuation of application No. PCT/US2019/051357, filed on Sep. 16, 2019.
Claims priority of provisional application 62/731,978, filed on Sep. 17, 2018.
Claims priority of provisional application 62/731,980, filed on Sep. 17, 2018.
Prior Publication US 2024/0384314 A1, Nov. 21, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 17/06 (2006.01); C12N 1/16 (2006.01); C12P 7/42 (2006.01)
CPC C12P 17/06 (2013.01) [C12N 1/16 (2013.01); C12P 7/42 (2013.01); C12Y 103/03006 (2013.01); C12Y 203/01206 (2015.07); C12Y 404/01026 (2015.07)] 19 Claims
 
1. A method of producing a fatty acyl-CoA in a peroxisome, the method comprising:
providing a fatty acid to a genetically modified microorganism, wherein the genetically modified organism is a yeast or a fungus, wherein the microorganism comprises a biosynthetic enzyme targeted to the peroxisome, wherein the biosynthetic enzyme is capable of catabolizing the fatty acid into the fatty acyl-CoA;
culturing the microorganism under conditions sufficient to produce the fatty acyl-CoA in the peroxisome; and
isolating the fatty acyl-CoA.