US 12,442,025 B2
Engineered polypeptides and its application in the synthesis of tyrosine or tyrosine derivatives
Haibin Chen, Ningbo (CN); Chengxiao Zhang, Ningbo (CN); Shumin Shi, Ningbo (CN); Baoqin Cai, Ningbo (CN); Lei Sun, Ningbo (CN); Qinli Peng, Ningbo (CN); Zikun Wang, Ningbo (CN); and Yong Koy Bong, Ningbo (CN)
Assigned to Enzymaster (NINGBO) Bio-Engineering Co., Ltd., Zhejiang (CN)
Appl. No. 17/440,954
Filed by Enzymaster (Ningbo) Bio-Engineering Co., Ltd, Ningbo (CN)
PCT Filed Mar. 30, 2020, PCT No. PCT/CN2020/082139
§ 371(c)(1), (2) Date Sep. 20, 2021,
PCT Pub. No. WO2020/207282, PCT Pub. Date Oct. 15, 2020.
Claims priority of application No. 201910274690.2 (CN), filed on Apr. 7, 2019.
Prior Publication US 2023/0010880 A1, Jan. 12, 2023
Int. Cl. C12N 9/88 (2006.01); C12N 15/70 (2006.01); C12P 13/04 (2006.01); C12P 13/22 (2006.01); C12N 15/10 (2006.01); C40B 40/08 (2006.01)
CPC C12P 13/04 (2013.01) [C12N 9/88 (2013.01); C12N 15/70 (2013.01); C12P 13/225 (2013.01); C12N 15/1034 (2013.01); C12Y 401/99002 (2013.01); C40B 40/08 (2013.01)] 22 Claims
 
1. An engineered polypeptide that catalyzes the synthesis of L-tyrosine in enantiomeric excess, said polypeptide comprising an amino acid sequence having at least one amino acid substitution relative to SEQ ID NO: 2, wherein said at least one amino acid substitution is selected from the group consisting of X132R, X354P, X454E, and combinations thereof, further wherein said amino acid sequence is selected from the group consisting of SEQ ID No: 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, and 308.