	US 12,442,022 B2
	Enzyme nanoparticles with methane oxidation activity
Jeewon Lee, Seoul (KR); and Hyun Jin Kim, Seoul (KR)
Assigned to Korea University Research and Business Foundation, Seoul (KR)
Appl. No. 17/599,153
Filed by Korea University Research and Business Foundation, Seoul (KR)
PCT Filed Mar. 20, 2020, PCT No. PCT/KR2020/003841 § 371(c)(1), (2) Date Sep. 28, 2021, PCT Pub. No. WO2020/204425, PCT Pub. Date Oct. 8, 2020.
Claims priority of application No. 10-2019-0036923 (KR), filed on Mar. 29, 2019.
Prior Publication US 2022/0170051 A1, Jun. 2, 2022
Int. Cl. C12P 7/04 (2006.01); C12N 9/02 (2006.01); C12N 9/96 (2006.01); C12N 11/04 (2006.01)

CPC C12P 7/04 (2013.01) [C12N 9/0071 (2013.01); C12N 9/96 (2013.01); C12N 11/04 (2013.01); C12Y 114/13025 (2013.01); C12Y 114/18003 (2013.01)]

3 Claims

1. An enzyme nanoparticle comprising:

a first active domain of a methane monooxygenase (MMO) comprising the amino acid sequence of SEQ ID NO: 2 and a second active domain of the MMO comprising the amino acid sequence of SEQ ID NO: 3;

and a human ferritin heavy-chain protein, wherein the first active domain and the second active domain are fused to a surface of the human ferritin heavy-chain protein, wherein the enzyme nanoparticle converts methane to methanol, and wherein the MMO and the human ferritin heavy-chain protein are linked by a peptide linker.