	US 12,442,012 B2
	Method and biological agent for catalyzing esterification of plant free carotenoids and transgenic plant
Ying Wang, Guangdong (CN); Shaohua Zeng, Guangdong (CN); and Shuang Lin, Guangdong (CN)
Assigned to SOUTH CHINA BOTANICAL GARDEN, CHINESE ACADEMY OF SCIENCES, Guangdong (CN)
Filed by SOUTH CHINA BOTANICAL GARDEN, CHINESE ACADEMY OF SCIENCES, Guangdong (CN)
Filed on Jan. 16, 2023, as Appl. No. 18/154,861.
Claims priority of application No. 202210192259.5 (CN), filed on Mar. 1, 2022.
Prior Publication US 2023/0295647 A1, Sep. 21, 2023
Int. Cl. C12N 15/82 (2006.01); C12N 9/10 (2006.01)

CPC C12N 15/825 (2013.01) [C12N 9/1029 (2013.01); C12Y 203/01 (2013.01)]

6 Claims

1. A method for catalyzing the esterification of plant free carotenoids, comprising the following steps:

(a) preparing host cells of a plant;

(b) transforming a nucleic acid molecule encoding a carotenoid esterase into the host cells of (a), wherein the nucleic acid molecule is: the LbZAT1 gene, the nucleotide sequence of which is SEQ ID NO:1 or a nucleic acid sequence encoding the amino acid sequence of SEQ ID NO:4; and

(c) culturing the transformed host cells of (b) overnight in the dark under humidity suitable to catalyze the esterification of plant free carotenoids.