	US 12,442,010 B2
	*Pod shatter tolerance in Brassica* plants**
Sarah Atwood, Ankeny, IA (US); Norbert Brugiere, Johnston, IA (US); Igor Falak, Guelph (CA); Kevin A Fengler, Clive, IA (US); Siva S. Ammiraju Jetty, Johnston, IA (US); and Jonathan Myrvold, Waukee, IL (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Appl. No. 17/617,724
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
PCT Filed Jun. 17, 2020, PCT No. PCT/US2020/038124 § 371(c)(1), (2) Date Dec. 9, 2021, PCT Pub. No. WO2020/257273, PCT Pub. Date Dec. 24, 2020.
Claims priority of provisional application 62/863,551, filed on Jun. 19, 2019.
Prior Publication US 2022/0298519 A1, Sep. 22, 2022
Int. Cl. C12Q 1/6895 (2018.01); C07K 14/415 (2006.01); C12N 9/22 (2006.01); C12N 9/24 (2006.01); C12N 15/11 (2006.01); C12N 15/82 (2006.01)

CPC C12N 15/8213 (2013.01) [C07K 14/415 (2013.01); C12N 9/22 (2013.01); C12N 9/24 (2013.01); C12N 15/111 (2013.01); C12Q 1/6895 (2013.01); C12N 2310/20 (2017.05)]

14 Claims

1. A method of identifying a Brassica plant, cell, or germplasm thereof comprising a BnIND-A genomic deletion that contributes to a pod shatter tolerance phenotype, the method comprising:

a. obtaining a nucleic acid sample from a Brassica plant cell, or germplasm; and

b. screening the sample for genomic sequence comprising a deletion of the BnIND-A gene on chromosome N03, wherein the deleted genomic segment results in a breakpoint locus corresponding to positions 10,002-10,003 of SEQ ID NO:2, and wherein the BnIND-A deletion contributes to pod shatter tolerance phenotype in Brassica.