US 12,442,008 B2
Microorganisms with enhanced L-branched-chain amino acids productivity and method for producing L-branched-chain amino acids using the same
Byoung Hoon Yoon, Seoul (KR); Hyo Jin Kim, Seoul (KR); Seon Hye Kim, Seoul (KR); Hyung Joon Kim, Seoul (KR); Sun Hyoung Choi, Seoul (KR); and Ji Hye Lee, Seoul (KR)
Assigned to CJ CHEILJEDANG CORPORATION, Seoul (KR)
Appl. No. 17/801,994
Filed by CJ CHEILJEDANG CORPORATION, Seoul (KR)
PCT Filed May 6, 2021, PCT No. PCT/KR2021/005641
§ 371(c)(1), (2) Date Aug. 24, 2022,
PCT Pub. No. WO2021/235742, PCT Pub. Date Nov. 25, 2021.
Claims priority of application No. 10-2020-0061174 (KR), filed on May 21, 2020.
Prior Publication US 2023/0086419 A1, Mar. 23, 2023
Int. Cl. C12N 15/77 (2006.01); C07K 14/34 (2006.01); C12P 13/06 (2006.01); C12P 13/08 (2006.01); C12R 1/15 (2006.01)
CPC C12N 15/77 (2013.01) [C07K 14/34 (2013.01); C12P 13/06 (2013.01); C12P 13/08 (2013.01); C12R 2001/15 (2021.05)] 20 Claims
 
1. A microorganism of the genus Corynebacterium for producing L-branched-chain amino acids, comprising a polynucleotide sequence represented by General Formula 1 below:
X—Z—Y—Z′  [General Formula 1]
in the General Formula 1 above,
X is SEQ ID NO: 1 or a sequence having a homology of 90% or more thereto,
Y is a transposon,
Z is the sequence from 0 to the nth nucleotide of SEQ ID NO: 5 or a sequence having a homology of 80% or more thereto, and Z′ is the sequence from the n+1th nucleotide of SEQ ID NO: 5 to the 188th nucleotide of SEQ ID NO: 5 or a sequence having a homology of 80% or more thereto,
wherein n is a natural number from 0 to 187.