	US 12,442,007 B2
	Method for producing target protein
Koichi Harazono, Kyoto (JP)
Assigned to Nagase Viita Co., Ltd., Okayama (JP)
Appl. No. 18/000,216
Filed by Nagase Viita Co., Ltd., Okayama (JP)
PCT Filed May 20, 2021, PCT No. PCT/JP2021/019197 § 371(c)(1), (2) Date Nov. 29, 2022, PCT Pub. No. WO2021/251098, PCT Pub. Date Dec. 16, 2021.
Claims priority of application No. 2020-102499 (JP), filed on Jun. 12, 2020.
Prior Publication US 2023/0203511 A1, Jun. 29, 2023
Int. Cl. C12N 9/16 (2006.01); C12N 9/24 (2006.01); C12N 15/76 (2006.01)

CPC C12N 15/76 (2013.01) [C12N 9/16 (2013.01); C12N 9/2405 (2013.01); C12Y 301/04004 (2013.01)]

5 Claims

1. A method for producing a target protein, comprising culturing cells transformed with a vector,

wherein the vector comprises a gene of the target protein and does not comprise an antibiotic resistance gene, a recombinase recognition sequence, nor a gene essential for cell survival,

wherein the target protein is phospholipase, glucanase, or protease, and

wherein the vector further comprises a replication initiator sequence obtained from pIJ702 or pIJ350.