US 12,441,996 B2
Use of DNA origami nanostructures for molecular information based data storage systems
Cherry Gupta, Columbus, OH (US); Craig M. Bartling, Powell, OH (US); Rachel R. Spurbeck, Columbus, OH (US); Anthony D. Duong, Columbus, OH (US); James Ha, Columbus, OH (US); Miguel D. Pedrozo, Columbus, OH (US); and Nickolas R. Andrioff, Columbus, OH (US)
Assigned to BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed by BATTELLE MEMORIAL INSTITUTE, Columbus, OH (US)
Filed on Dec. 6, 2024, as Appl. No. 18/972,564.
Claims priority of provisional application 63/607,741, filed on Dec. 8, 2023.
Prior Publication US 2025/0188449 A1, Jun. 12, 2025
Int. Cl. C12N 15/10 (2006.01); C12Q 1/6869 (2018.01)
CPC C12N 15/1093 (2013.01) [C12Q 1/6869 (2013.01)] 20 Claims
 
1. A library comprising a plurality of origami folded DNA data storage files (DNAFiles), each of said DNAFiles comprising
a single stranded DNA scaffold; and
a plurality of single stranded DNA staple oligonucleotides that each bind through complementary base pairing with two non-contiguous nucleic acid sequences of the DNA scaffold, wherein said staple oligonucleotides cause the DNA scaffold to fold into a two or three dimensional shape having a first surface;
a plurality of data oligonucleotides, said data oligonucleotides comprising a sequence complementary to a nucleic acid sequence of said single stranded DNA scaffold, a nucleic acid sequence that encodes digital information, a first primer binding sequence and a second primer binding sequence, wherein the first primer binding sequence is 5′ to the digital information encoding nucleic acid sequence, and the second primer binding sequence is 3′ to the digital information encoding nucleic acid sequence, wherein said plurality of data oligonucleotides are localized to said first surface, wherein the individual DNAFiles differ from one another based on the nucleic acid sequences of the plurality of data oligonucleotides bound to the DNA scaffold of each DNAFile.