	US 12,441,993 B2
	Isolation of extracellular vesicles by precipitation or immobilization using polyethylenimine and polyethylenimine-coated solid supports
Catherine Taylor, Boundary Creek (CA); and Jeremy Roy, Moncton (CA)
Assigned to ATLANTIC CANCER RESEARCH INSTITUTE, Moncton (CA)
Appl. No. 17/052,643
Filed by Atlantic Cancer Research Institute (ACRI), Moncton (CA)
PCT Filed May 17, 2019, PCT No. PCT/CA2019/050669 § 371(c)(1), (2) Date Nov. 3, 2020, PCT Pub. No. WO2019/218077, PCT Pub. Date Nov. 21, 2019.
Claims priority of provisional application 62/673,415, filed on May 18, 2018.
Prior Publication US 2021/0238584 A1, Aug. 5, 2021
Int. Cl. C12N 15/10 (2006.01); A61K 35/51 (2015.01); A61K 35/545 (2015.01); G01N 33/574 (2006.01)

CPC C12N 15/1013 (2013.01) [A61K 35/51 (2013.01); A61K 35/545 (2013.01); G01N 33/57488 (2013.01)]

14 Claims

1. A method for the removal of extracellular vesicle(s) (EV)s from a sample containing EVs, comprising:

(i) contacting the sample with a solution of polyethyleneimines to form EV-polyethylenimine complexes; and

(ii) precipitating out the EV-polyethylenimine complexes to remove the EVs from the sample,

wherein the polyethylenimines are present in the solution at a concentration of from about 1 μg/mL to about 5 mg/mL, the solution of polyethylenamines is the only precipitating agent used in the method and the EVs are from a biological sample.