Gordana Vunjak-Novakovic, New York, NY (US); Keith Yeager, Springfield, NJ (US); Kacey Ronaldson, New York, NY (US); Stephen Ma, Layton, UT (US); and Timothy Chen, New York, NY (US)
Assigned to The Trustees of Columbia University in the City of New York, New York, NY (US)
Filed by THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, New York, NY (US)
Filed on Feb. 17, 2022, as Appl. No. 17/674,196.
Application 17/674,196 is a continuation of application No. 15/151,751, filed on May 11, 2016, granted, now 11,299,714.
Claims priority of provisional application 62/275,385, filed on Jan. 6, 2016.
Claims priority of provisional application 62/198,502, filed on Jul. 29, 2015.
Claims priority of provisional application 62/159,953, filed on May 11, 2015.
Prior Publication US 2022/0315902 A1, Oct. 6, 2022
1. An engineered three-dimensional micro-tissue comprising a cell-hydrogel construct comprising electromechanically conditioned cardiomyocytes and fibroblasts encapsulated in a hydrogel wherein the electromechanically conditioned cardiomyocytes are mature cardiomyocytes including sarcomeres and a network of T-tubules, and wherein the electromechanical conditioning comprises subjecting the cell-hydrogel construct to electrical stimulation that increases in intensity over a time period, wherein the electrical stimulation results in the mature cardiomyocytes including sarcomeres and the network of T-tubules.