US 12,441,981 B2
Extracellular matrix scaffolds
Rachelle Simko, Pittsburgh, PA (US); and Adam Feinberg, Pittsburgh, PA (US)
Assigned to Carnegie Mellon University, Pittsburgh, PA (US)
Filed by Carnegie Mellon University, Pittsburgh, PA (US)
Filed on Nov. 24, 2021, as Appl. No. 17/535,094.
Application 17/535,094 is a continuation of application No. 15/610,125, filed on May 31, 2017, abandoned.
Claims priority of provisional application 62/392,389, filed on May 31, 2016.
Prior Publication US 2022/0333063 A1, Oct. 20, 2022
Int. Cl. C12N 5/00 (2006.01); A61L 27/16 (2006.01); A61L 27/18 (2006.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/50 (2006.01)
CPC C12N 5/0062 (2013.01) [A61L 27/16 (2013.01); A61L 27/18 (2013.01); A61L 27/3633 (2013.01); A61L 27/38 (2013.01); A61L 27/50 (2013.01); C12N 5/0012 (2013.01); C12N 5/0068 (2013.01); C12N 2531/00 (2013.01); C12N 2533/90 (2013.01); C12N 2535/00 (2013.01); C12N 2539/10 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A micro-tissue raft comprising:
a cell patch comprising binders exposed on a first side of the cell patch, wherein cells of the cell patch comprise a Zonula Occludens-1 (ZO-1) protein expression that maintains tight cell-cell junctions; and
an extracellular matrix compound comprising at least two cell types, the extracellular matrix being attached to a second side of the cell patch and that forms a base for the cell patch, wherein the extracellular matrix compound is configured to be responsive to one or more tension forces expressed by the cell patch, wherein the one or more tension forces cause the extracellular matrix compound to fold into a specified geometry around the cell patch, wherein the specified geometry substantially conceals the expressed binders of the cell patch, wherein the ZO-1 protein expression assists folding of the extracellular matrix around the cell patch.