US 12,441,980 B2
Methods for overcoming glutamine deprivation during mammalian cell culture
William C. Yang, Cary, NC (US); Yao-Ming Huang, Cary, NC (US); Marty Sinacore, Andover, MA (US); Thomas Ryll, Weston, MA (US); Jiuyi Lu, Ipswich, MA (US); Jaesun Lee, Incheon (KR); Kyngju Lee, Incheon (KR); Hyoung Taek Lim, Incheon (KR); and Eunchong Yang, Seoul (KR)
Assigned to Biogen MA Inc., Cambridge, MA (US); and Samsung Bioepis, Yeonsu-gu (KR)
Filed by Biogen MA Inc., Cambridge, MA (US); and Samsung Bioepis, Yeonsu-gu (KR)
Filed on Aug. 13, 2021, as Appl. No. 17/401,558.
Application 17/401,558 is a continuation of application No. 15/128,732, granted, now 11,124,760, previously published as PCT/US2015/022259, filed on Mar. 24, 2015.
Claims priority of provisional application 61/969,800, filed on Mar. 24, 2014.
Prior Publication US 2022/0213431 A1, Jul. 7, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); C12P 21/00 (2006.01)
CPC C12N 5/0018 (2013.01) [C12P 21/00 (2013.01); C12N 2500/40 (2013.01); C12N 2500/60 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method of producing a polypeptide of interest in a glutamine-free cell culture medium, the method comprising:
culturing cells expressing the polypeptide of interest in the glutamine-free cell culture medium under conditions that support expression of the polypeptide of interest,
wherein the glutamine-free cell culture medium comprises: (i) between about 20 μM and about 100 μM hypoxanthine; (ii) between about 15 μM and about 20 μM thymidine; and (iii) adenosine and/or at least one corticosteroid, and
wherein the cells are initially maintained in a culture containing glutamine and are transitioned to the glutamine-free cell culture medium for production phase.