US 12,441,977 B2
Lactic acid bacteria
Anais Jedrzejowski, Dangé-Saint-Romain (FR); Christophe Fremaux, Dangé-Saint-Romain (FR); Sabine Van Dillen, Dangé-Saint-Romain (FR); Thomas Desfougeres, Dangé-Saint-Romain (FR); Max Charles Jodeau, Dangé-Saint-Romain (FR); and Damien Lugand, Dangé-Saint-Romain (FR)
Assigned to International N&H Denmark ApS, Kongens Lyngby (DK)
Appl. No. 17/437,255
Filed by International N&H Denmark APS, Kongens Lyngby (DK)
PCT Filed Mar. 13, 2020, PCT No. PCT/EP2020/056766
§ 371(c)(1), (2) Date Sep. 8, 2021,
PCT Pub. No. WO2020/182976, PCT Pub. Date Sep. 17, 2020.
Claims priority of application No. 19162856 (EP), filed on Mar. 14, 2019; application No. 201910406044.7 (CN), filed on May 16, 2019; and application No. 19214119 (EP), filed on Dec. 6, 2019.
Prior Publication US 2022/0169974 A1, Jun. 2, 2022
Int. Cl. C12N 1/20 (2006.01); A23C 9/123 (2006.01); A23L 13/74 (2023.01); C12N 15/74 (2006.01); C12P 1/04 (2006.01); G01N 33/50 (2006.01)
CPC C12N 1/20 (2013.01) [A23C 9/1238 (2013.01); A23L 13/74 (2016.08); C12N 15/746 (2013.01); C12P 1/04 (2013.01); G01N 33/5038 (2013.01); A23V 2400/249 (2023.08)] 16 Claims
 
1. A polynucleotide encoding a β-galactosidaseFS, which comprises an amino acid sequence which has at least 95% identity with SEQ ID NO:2; and which is defined as a lacZ allele which increases the ratio of the activity of lactose importation of the LacS permease at pH 4.5 over the activity of lactose hydrolysis of the beta-galactosidase at pH 4.5 above 8 in a DGCC715 derivative, said DGCC715 derivative being a strain DGCC715 (deposited at the DSMZ on Feb. 12, 2019 under the accession number DSM33036), into which its lacZ gene was replaced by said polynucleotide encoding a β-galactosidaseFS.