US 12,441,809 B2
Affinity chromatography media for removal of anti-a and/or anti-b antibodies
Nanying Bian, Lexington, MA (US); Chia-Yun Sun, Arlington, MA (US); Melissa Holstein, Concord, MA (US); Kristen Cotoni, Malden, MA (US); Matthew T. Stone, Arlington, MA (US); and Santosh Rahane, Acton, MA (US)
Assigned to Merck Patent GmbH, Darmstadt (DE)
Filed by Merck Patent GmbH, Darmstadt (DE)
Filed on Nov. 4, 2020, as Appl. No. 17/088,659.
Application 17/088,659 is a continuation of application No. 15/226,461, filed on Aug. 2, 2016, abandoned.
Claims priority of provisional application 62/215,401, filed on Sep. 8, 2015.
Prior Publication US 2021/0047430 A1, Feb. 18, 2021
Int. Cl. C07K 16/34 (2006.01); B01D 15/20 (2006.01); B01D 15/38 (2006.01); B01D 15/42 (2006.01); B01J 20/285 (2006.01); B01J 20/289 (2006.01); B01J 20/32 (2006.01); C07K 1/22 (2006.01)
CPC C07K 16/34 (2013.01) [B01D 15/20 (2013.01); B01D 15/3809 (2013.01); B01D 15/426 (2013.01); B01J 20/285 (2013.01); B01J 20/289 (2013.01); B01J 20/321 (2013.01); B01J 20/3274 (2013.01); C07K 1/22 (2013.01); B01J 2220/54 (2013.01); B01J 2220/58 (2013.01); C07K 2317/21 (2013.01); C07K 2317/70 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of removing anti-B antibodies from a sample, the method comprising the steps of:
(a) providing a sample comprising an amount of anti-B antibodies; incubating the sample with a media for removing anti-B antibodies from a sample, the media comprising a solid support with a blood group B antigen oligosaccharide based ligand attached thereto, wherein the oligosaccharide based ligand is attached to the solid support at a ligand loading of at least 0.8 mg/ml of solid support, wherein the blood group B antigen ligand has the following structure:

OG Complex Work Unit Chemistry
and wherein the media is stable under acid and alkaline conditions and wherein the solid support comprises a polyvinyl ether polymer and the blood group B antigen oligosaccharide based ligands are attached via pAA tentacle chemistry to a solid support, for the media to bind blood group B antibodies;
(b) recovering portion of the sample which is not bound to the media; and
(c) measuring amount of anti-B antibodies in the portion of the sample in (b), wherein the amount of anti-B antibodies in (c) is at least 80% less than the amount of anti-B antibodies in the sample in (a), wherein the media can be readily eluted and cleaned in place multiple times under a wide range of pH conditions, without losing its capability to remove the anti-B antibodies from the sample.