US 12,441,771 B2
Method for purifying pea albumin PA1a by using negatively charged polysaccharide
Xingfei Li, Wuxi (CN); Yufei Hua, Wuxi (CN); Jie Long, Wuxi (CN); Caimeng Zhang, Wuxi (CN); Shunuan Yang, Wuxi (CN); Yeming Chen, Wuxi (CN); and Xiangzhen Kong, Wuxi (CN)
Appl. No. 17/634,557
Filed by JIANGNAN UNIVERSITY, Wuxi (CN)
PCT Filed Jul. 14, 2021, PCT No. PCT/CN2021/106242
§ 371(c)(1), (2) Date Feb. 11, 2022,
PCT Pub. No. WO2022/028221, PCT Pub. Date Feb. 10, 2022.
Claims priority of application No. 202010771533.5 (CN), filed on Aug. 4, 2020.
Prior Publication US 2023/0151066 A1, May 18, 2023
Int. Cl. C07K 1/14 (2006.01); C07K 14/415 (2006.01); C07K 1/30 (2006.01); C07K 1/32 (2006.01)
CPC C07K 14/415 (2013.01) [C07K 1/14 (2013.01); C07K 1/30 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for purifying pea albumin PA1a by using a negatively charged polysaccharide, said method comprising combining pea whey as a raw material and a natural edible negatively charged polysaccharide as a recycling material, said pea whey having PA1a as a targeted protein; said negatively charged polysaccharide preferentially binds to non-target proteins of said pea whey and is precipitated under complex coacervation conditions to obtain a polysaccharide-PA2/lectin complex coacervate precipitate and removed from the pea whey; and, from the remaining pea whey, the targeted protein, pea albumin PA1a is purified.