US 12,441,753 B2
Methods for purifying steviol glycosides and uses of the same
Avetik Markosyan, Yerevan (AM)
Assigned to PureCircle Sdn. Bhd., Negeri Sembilan (MY); and The Coca-Cola Company, Atlanta, GA (US)
Appl. No. 14/362,275
Filed by Avetik Markosyan, Yerevan (AM)
PCT Filed Dec. 19, 2012, PCT No. PCT/US2012/070562
§ 371(c)(1), (2) Date Jun. 2, 2014,
PCT Pub. No. WO2013/096420, PCT Pub. Date Jun. 27, 2013.
Application 14/362,275 is a continuation in part of application No. 13/580,098, filed on Nov. 6, 2012, granted, now 8,981,081.
Claims priority of provisional application 61/651,099, filed on May 24, 2012.
Claims priority of provisional application 61/577,202, filed on Dec. 19, 2011.
Prior Publication US 2014/0343262 A1, Nov. 20, 2014
Prior Publication US 2016/0031924 A9, Feb. 4, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. A23L 2/00 (2006.01); A23L 2/60 (2006.01); A23L 27/30 (2016.01); A23L 29/30 (2016.01); A23L 33/125 (2016.01); B01D 15/18 (2006.01); C07H 1/08 (2006.01); C07H 15/24 (2006.01)
CPC C07H 15/24 (2013.01) [A23L 2/00 (2013.01); A23L 2/60 (2013.01); A23L 27/30 (2016.08); A23L 27/33 (2016.08); A23L 27/34 (2016.08); A23L 27/36 (2016.08); A23L 29/30 (2016.08); A23L 29/37 (2016.08); A23L 33/125 (2016.08); B01D 15/1871 (2013.01); C07H 1/08 (2013.01); A23V 2002/00 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method for separating Reb X from other steviol glycosides comprising the steps of:
a. passing a solution of steviol glycosides comprising Reb X through a multi-column system including a plurality of consecutively connected columns packed with an macroporous polymeric adsorbent resin to provide at least two consecutive columns having steviol glycosides adsorbed onto the macroporous polymeric adsorbent resin, wherein the steviol glycosides separate into different portions of the at least two consecutive columns, resulting in different individual glycoside contents in different portions; and
b. eluting the adsorbed steviol glycosides from at least one column having a higher Reb X content compared to any other column using an alcoholic solvent to provide an eluted solution comprising Reb X which has been separated from the other steviol glycosides in the solution of steviol glycosides.