	US 12,441,751 B2
	Metabolic labeling and molecular enhancement of biological materials using bioorthogonal reactions
Harald C. Ott, Wenham, MA (US); Xi Ren, Malden, MA (US); Jordan P. Bloom, Charlestown, MA (US); and Taufiek Konrad Rajab, Hirrlingen (DE)
Assigned to The General Hospital Corporation, Boston, MA (US)
Filed by The General Hospital Corporation, Boston, MA (US)
Filed on Mar. 8, 2023, as Appl. No. 18/118,909.
Application 18/118,909 is a division of application No. 15/624,399, filed on Jun. 15, 2017, granted, now 11,634,448.
Claims priority of provisional application 62/350,259, filed on Jun. 15, 2016.
Prior Publication US 2023/0287030 A1, Sep. 14, 2023
Int. Cl. A61K 31/655 (2006.01); A61K 31/7004 (2006.01); A61K 35/22 (2015.01); A61K 35/34 (2015.01); A61K 35/36 (2015.01); A61K 35/407 (2015.01); A61K 35/42 (2015.01); A61K 35/44 (2015.01); A61L 27/36 (2006.01); A61L 27/38 (2006.01); A61L 27/54 (2006.01); A61L 27/56 (2006.01); C07H 13/04 (2006.01); C12N 5/07 (2010.01)

CPC C07H 13/04 (2013.01) [A61K 31/655 (2013.01); A61K 35/22 (2013.01); A61K 35/34 (2013.01); A61K 35/36 (2013.01); A61K 35/407 (2013.01); A61K 35/42 (2013.01); A61K 35/44 (2013.01); A61L 27/3633 (2013.01); A61L 27/3808 (2013.01); A61L 27/3813 (2013.01); A61L 27/3826 (2013.01); A61L 27/383 (2013.01); A61L 27/54 (2013.01); C12N 5/06 (2013.01); A61L 2300/256 (2013.01); A61L 2300/406 (2013.01); A61L 2300/414 (2013.01); A61L 2300/42 (2013.01)]

11 Claims

1. A method of functionalizing an extracellular matrix of an organ or tissue of a mammal, the method comprising:

i. harvesting the organ or tissue;

ii. culturing the organ or tissue using media comprising a nutrient functionalized with a chemical group that is reactive in a biorthogonal chemical reaction; and

iii. decellularizing the organ or tissue to obtain a decellularized scaffold comprising the extracellular matrix functionalized with the chemical group that is reactive in the biorthogonal chemical reaction.