	US 12,441,643 B2
	Anaerobic-aerobic bioremediation of contaminated water
Shaily Mahendra, Santa Monica, CA (US); and Alexandra Polasko, Los Angeles, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Appl. No. 16/766,582
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
PCT Filed Nov. 21, 2018, PCT No. PCT/US2018/062215 § 371(c)(1), (2) Date May 22, 2020, PCT Pub. No. WO2019/104144, PCT Pub. Date May 31, 2019.
Claims priority of provisional application 62/590,030, filed on Nov. 22, 2017.
Prior Publication US 2020/0369546 A1, Nov. 26, 2020
Int. Cl. C02F 3/34 (2023.01); B09C 1/00 (2006.01); B09C 1/10 (2006.01); C02F 3/30 (2023.01); C12N 1/20 (2006.01); C02F 101/32 (2006.01); C02F 101/34 (2006.01); C02F 101/36 (2006.01); C02F 103/00 (2006.01); C02F 103/06 (2006.01)

CPC C02F 3/341 (2013.01) [B09C 1/002 (2013.01); C02F 3/30 (2013.01); C12N 1/20 (2013.01); B09C 1/10 (2013.01); C02F 2101/322 (2013.01); C02F 2101/34 (2013.01); C02F 2101/36 (2013.01); C02F 2103/007 (2013.01); C02F 2103/06 (2013.01)]

18 Claims

1. A method for bioremediation of a contaminated substance, the substance being contaminated with a halogenated volatile organic compound (HVOC) and a HVOC stabilizer compound, the method comprising contacting the contaminated substance under anaerobic conditions with a microbial mixture in an amount sufficient to lower the concentration of the contaminating HVOC and HVOC stabilizer compounds in the contaminated substance, wherein the microbial mixture comprises (a) Pseudonocardia dioxanivorans CB1190 and (b) Dehalococcoides, Desulfitobacterium, Dehalobacter, Dehalogenimonas, Dehalospirillum, Desulfuromonas, Geobacter, Clostridium, or Enterobacter bacteria.