US 12,440,844 B2
Loop-mediated isothermal amplification devices and systems for detecting genetic material
Markus Kalkum, Duarte, CA (US); Daniel Roeth, Duarte, CA (US); Isaac Bishara, Duarte, CA (US); Miro Rusnak, Duarte, CA (US); and Sanjeet Dadwal, Duarte, CA (US)
Assigned to City of Hope, Duarte, CA (US)
Filed by City of Hope, Duarte, CA (US)
Filed on Dec. 22, 2021, as Appl. No. 17/559,706.
Claims priority of provisional application 63/129,888, filed on Dec. 23, 2020.
Prior Publication US 2022/0193658 A1, Jun. 23, 2022
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6806 (2018.01); G01K 3/00 (2006.01); G01N 21/64 (2006.01); G06V 10/56 (2022.01); G06V 20/69 (2022.01)
CPC B01L 3/508 (2013.01) [C12Q 1/6806 (2013.01); G01K 3/005 (2013.01); G01N 21/6428 (2013.01); G01N 21/6456 (2013.01); G06V 10/56 (2022.01); G06V 20/693 (2022.01); B01L 2200/10 (2013.01); B01L 2200/147 (2013.01); B01L 2200/16 (2013.01); B01L 2200/18 (2013.01); B01L 2300/046 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/1827 (2013.01); G01N 2201/062 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A device configured to conduct loop-mediated isothermal amplification, the device comprising:
an enclosure having outer walls configured to define an internal space, the enclosure configured to isolate the internal space from light outside the enclosure;
a tray assembly situated within the internal space, the tray assembly configured to support a reaction vessel configured for holding a sample for amplification, wherein the tray assembly includes a center plate interposed between a plurality of mica plates and wherein the plurality of mica plates have brass connector lip for a slide-on power connector and wherein the center plate is a copper plate;
a plurality of light emitters situated inside the enclosure, the plurality of light emitters configured to illuminate the reaction vessel for exciting a fluorescence of the sample in the reaction vessel;
an imaging device situated in the internal space and configured to obtain an image of the sample for determining a quantity of the fluorescence in the sample; and
a controller communicatively coupled to the tray assembly, the plurality of light emitters, and the imaging device, the controller configured to regulate a temperature of the tray assembly and instruct the imaging device to obtain the image for determining the quantity of the fluorescence in the sample.