US 12,440,841 B2
Microfabricated droplet dispensor with immiscible fluid and genetic sequencer
John S Foster, New Orleans, LA (US); Mehran Hoonejani, Goleta, CA (US); Kevin Shields, Santa Barbara, CA (US); Hansueli Meyer, Acton, MA (US); Robert Pinard, Lowell, MA (US); and Matthias Wahl, Solingen (DE)
Assigned to Owl biomedical, Inc., Goleta, CA (US)
Filed by Owl biomedical, Inc., Goleta, CA (US)
Filed on May 19, 2021, as Appl. No. 17/324,570.
Application 17/324,570 is a continuation in part of application No. 16/009,163, filed on Jun. 14, 2018, granted, now 11,040,347.
Prior Publication US 2021/0268506 A1, Sep. 2, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. B01L 3/00 (2006.01); C12Q 1/6869 (2018.01)
CPC B01L 3/502784 (2013.01) [B01L 3/502738 (2013.01); B01L 3/502761 (2013.01); C12Q 1/6869 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0673 (2013.01); B01L 2300/021 (2013.01); B01L 2400/0622 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A system for preparing and analyzing genetic material, comprising:
 a microfluidic channel formed in a substrate;
a first fluid, including at least one target particle and at least one bead and non-target material;
a microfabricated MEMS fluidic valve, configured to open and close the microfluidic channel and formed in the same substrate wherein the MEMS valve when in the sort position, separates the target particle and redirects the target particle into a first sort channel containing the first fluid;
a second fluid, immiscible with the first fluid;
a second microfluidic channel containing the second immiscible fluid
a nozzle disposed between the first sort channel and the second microfluidic channel, wherein the nozzle forms a droplet comprising a quantity of the first fluid along with the target particle, the quantity determined by the MEMS fluidic valve opening and closing,
a fluidic manifold that accepts the droplet and lyses the target particle enclosed within the particle to release genomic material; and
a sequencer that sequences the genomic material.