US 12,440,839 B2
High-throughput system and method for the temporary permeabilization of cells using lipid bilayers
Jason N. Belling, Los Angeles, CA (US); Steven J. Jonas, Los Angeles, CA (US); Joshua A. A. Jackman, Bradenton, FL (US); Nam-Joon Cho, Singapore (SG); and Paul S. Weiss, Los Angeles, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and NANYANG TECHNOLOGICAL UNIVERSITY, Singapore (SG)
Appl. No. 17/269,985
Filed by THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US); and NANYANG TECHNOLOGICAL UNIVERSITY, Singapore (SG)
PCT Filed Aug. 21, 2019, PCT No. PCT/US2019/047518
§ 371(c)(1), (2) Date Feb. 19, 2021,
PCT Pub. No. WO2020/041480, PCT Pub. Date Feb. 27, 2020.
Claims priority of provisional application 62/720,734, filed on Aug. 21, 2018.
Prior Publication US 2021/0346889 A1, Nov. 11, 2021
Int. Cl. B01L 3/00 (2006.01); C12N 15/90 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); C12N 15/907 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/16 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A microfluidic device for processing cells comprising:
one or more microchannels disposed in a substrate or chip and fluidically coupled to an inlet configured to receive a solution containing the cells along with molecules or other cargo to be delivered intracellularly to the cells, each of the one or more microchannels containing a constriction region therein having a width within the range of about 4 μm to about 10 μm, wherein the one or more microchannels and the respective constriction regions have a lipid bilayer formed on internal surfaces thereof.