US 12,440,838 B2
Multiwell dynamic model for a tumor-immune microenvironment
Jeffrey Borenstein, Newton, MA (US); Nathan Moore, Canton, MA (US); Daniel Doty, Arlington, MA (US); Timothy Haggerty, Concord, MA (US); Joseph Charest, Jamaica Plain, MA (US); Alla Gimbel, Medford, MA (US); Vienna Mott, Cambridge, MA (US); Brett Isenberg, Newton, MA (US); Hesham Azizgolshani, Belmont, MA (US); Brian Cain, Cambridge, MA (US); and Mark Mescher, West Newton, MA (US)
Assigned to The Charles Stark Draper Laboratory Inc., Cambridge, MA (US)
Filed by The Charles Stark Draper Laboratory Inc., Cambridge, MA (US)
Filed on Dec. 1, 2020, as Appl. No. 17/108,475.
Claims priority of provisional application 62/942,414, filed on Dec. 2, 2019.
Prior Publication US 2021/0162416 A1, Jun. 3, 2021
Int. Cl. B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 3/00 (2006.01); G01N 33/50 (2006.01)
CPC B01L 3/502761 (2013.01) [B01L 3/50273 (2013.01); G01N 33/5011 (2013.01); B01L 2200/0647 (2013.01); B01L 2300/047 (2013.01); B01L 2300/0829 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/168 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A microfluidic device, comprising:
a well plate comprising a plurality of wells, the well plate defining a plurality of microenvironment units fluidically coupled with the plurality of wells, each microenvironment unit comprising:
one or more compartments; and
a trapping feature positioned within a compartment of the one or more compartments, the trapping feature extending from a floor of the compartment and configured to trap a tissue sample introduced into the one or more compartments between a top surface of the trapping feature and a ceiling of the compartment, and to allow fluid to flow through the compartment at least between the tissue sample and the floor of the compartment; and
a plurality of micropumps each configured to control movement of a fluid sample through a respective well of the plurality of wells.