US 12,440,605 B2
Method for producing regenerated hair follicle primordium
Takashi Tsuji, Wako (JP); Yutaka Mori, Kyoto (JP); and Koh-ei Toyoshima, Tokyo (JP)
Assigned to ORGAN TECHNOLOGIES, INC., Tokyo (JP); and RIKEN, Wako (JP)
Appl. No. 16/972,103
Filed by ORGAN TECHNOLOGIES, INC., Tokyo (JP); and RIKEN, Wako (JP)
PCT Filed May 31, 2019, PCT No. PCT/JP2019/021690
§ 371(c)(1), (2) Date Dec. 4, 2020,
PCT Pub. No. WO2019/235375, PCT Pub. Date Dec. 12, 2019.
Claims priority of application No. 2018-106626 (JP), filed on Jun. 4, 2018.
Prior Publication US 2021/0196861 A1, Jul. 1, 2021
Int. Cl. A61K 36/00 (2006.01); A61L 27/38 (2006.01); C12N 5/071 (2010.01); C12N 5/0775 (2010.01)
CPC A61L 27/3895 (2013.01) [A61L 27/3886 (2013.01); C12N 5/0627 (2013.01); C12N 5/0666 (2013.01); A61L 2430/18 (2013.01)] 16 Claims
 
1. A method for producing regenerated hair follicle primordia, comprising:
forming a first cell aggregate in which epithelial cells are aggregated as a first population of cells;
forming a second cell aggregate in which mesenchymal cells are aggregated as a second population of cells;
arranging a guide in the first cell aggregate;
obtaining regenerated hair follicle primordia by culturing the first cell aggregate including the first population of epithelial cells and the second cell aggregate including the second population of mesenchymal cells while allowing them to be in contact,
wherein
the first population of epithelial cells is a population of cells in which cultured cells derived from bulge region epithelium have been treated into single cells;
the second population of mesenchymal cells is a group of cells in which cultured cells derived from dermal papillae have been treated into single cells;
the guide is placed at a position where the first cell aggregate is formed before or during the formation process; and
a culture support is not used when contacting the second cell population or the second cell aggregate with the first cell aggregate.