US 12,440,578 B2
Nucleic acid constructs comprising gene editing multi-sites and uses thereof
Sicco Hans Popma, Chalfont, PA (US); and Di Zhang, Chalfont, PA (US)
Assigned to IO BIOSCIENCES, INC., Chalfont, PA (US)
Appl. No. 16/486,804
Filed by IO BIOSCIENCES, INC., Chalfont, PA (US)
PCT Filed Feb. 22, 2018, PCT No. PCT/US2018/019297
§ 371(c)(1), (2) Date Aug. 16, 2019,
PCT Pub. No. WO2018/156818, PCT Pub. Date Aug. 30, 2018.
Claims priority of provisional application 62/573,353, filed on Oct. 17, 2017.
Claims priority of provisional application 62/551,383, filed on Aug. 29, 2017.
Claims priority of provisional application 62/538,328, filed on Jul. 28, 2017.
Claims priority of provisional application 62/461,991, filed on Feb. 22, 2017.
Prior Publication US 2019/0381192 A1, Dec. 19, 2019
Int. Cl. A61K 35/12 (2015.01); A61K 35/17 (2025.01); A61K 35/28 (2015.01); A61K 38/00 (2006.01); A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61K 48/00 (2006.01); C07K 14/00 (2006.01); C07K 16/28 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/62 (2006.01); C12N 15/63 (2006.01); C12N 15/66 (2006.01); C12N 15/85 (2006.01); C12N 15/90 (2006.01)
CPC A61K 48/005 (2013.01) [A61K 40/15 (2025.01); A61K 40/31 (2025.01); A61K 40/4211 (2025.01); C07K 16/2803 (2013.01); C12N 15/102 (2013.01); C12N 15/85 (2013.01); C12N 15/907 (2013.01); A61K 2239/31 (2023.05); A61K 2239/48 (2023.05); C07K 2317/622 (2013.01); C07K 2319/03 (2013.01); C07K 2319/33 (2013.01); C12N 2310/20 (2017.05)] 18 Claims
 
1. An engineered gene editing multi-site (GEMS) polynucleotide construct for insertion into a genome at an insertion site, wherein said engineered GEMS polynucleotide construct comprises:
a GEMS polynucleotide sequence comprising a plurality of nuclease recognition sequences, wherein at least one of said plurality of nuclease recognition sequences comprises the nucleotide sequence of SEQ ID NO: 14.