	US 12,440,558 B2
	Soybean allergy antigen
Kayoko Matsunaga, Aichi (JP); Akiko Yagami, Aichi (JP); Naoshi Shimojo, Aichi (JP); Masashi Nakamura, Aichi (JP); and Nayu Sato, Aichi (JP)
Assigned to HOYU CO., LTD., Aichi (JP)
Filed by HOYU CO., LTD., Aichi (JP)
Filed on Sep. 7, 2023, as Appl. No. 18/462,987.
Application 18/462,987 is a division of application No. 16/081,032, abandoned, previously published as PCT/JP2017/008593, filed on Mar. 3, 2017.
Claims priority of application No. 2016-041547 (JP), filed on Mar. 3, 2016.
Prior Publication US 2024/0123063 A1, Apr. 18, 2024
Int. Cl. A61K 39/35 (2006.01); A23L 11/30 (2016.01); A61K 49/00 (2006.01); A61P 37/08 (2006.01); C07K 4/10 (2006.01); C07K 14/415 (2006.01); C07K 14/47 (2006.01); G01N 33/53 (2006.01); A61K 38/00 (2006.01)

CPC A61K 39/35 (2013.01) [A23L 11/30 (2016.08); A61K 49/00 (2013.01); A61K 49/0006 (2013.01); A61P 37/08 (2018.01); C07K 4/10 (2013.01); C07K 14/415 (2013.01); C07K 14/47 (2013.01); G01N 33/53 (2013.01); A61K 38/00 (2013.01); G01N 2800/24 (2013.01)]

5 Claims

1. A method for detecting binding between a soybean antigen and an antibody that specifically binds to the soybean antigen present in a sample,

wherein the method uses a kit that comprises the soybean antigen,

wherein the soybean antigen is a Bowman-Birk type proteinase inhibitor D-II antigen comprising the amino acid sequence of SEQ ID NO: 2,

wherein the soybean antigen specifically binds to IgE antibody from a patient having allergy to soybean, wherein the soybean antigen is immobilized to a carrier or a surface,

wherein the method comprises:

(i) contacting the antibody in the sample with the soybean antigen from the kit, and

(ii) detecting binding between said soybean antigen and the antibody in the sample.