	US 12,440,552 B2
	Methods for immunizing pre-immune subjects against respiratory syncytial virus (RSV)
Trudy G Morrison, Northborough, MA (US)
Assigned to UNIVERSITY OF MASSACHUSETTS, Westborough, MA (US)
Filed by UNIVERSITY OF MASSACHUSETTS, Boston, MA (US)
Filed on Nov. 9, 2023, as Appl. No. 18/505,534.
Application 18/505,534 is a continuation of application No. 17/677,399, filed on Feb. 22, 2022, granted, now 11,813,324.
Application 17/677,399 is a continuation of application No. 17/028,288, filed on Sep. 22, 2020, abandoned.
Application 17/028,288 is a continuation of application No. 16/339,219, granted, now 10,842,862, issued on Nov. 24, 2020, previously published as PCT/US2017/052247, filed on Sep. 19, 2017.
Claims priority of provisional application 62/403,229, filed on Oct. 3, 2016.
Prior Publication US 2024/0082386 A1, Mar. 14, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 39/17 (2006.01); A61K 39/12 (2006.01); A61P 31/14 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)

CPC A61K 39/17 (2013.01) [A61K 39/12 (2013.01); A61P 31/14 (2018.01); C12N 7/00 (2013.01); A61K 2039/5256 (2013.01); A61K 2039/5258 (2013.01); C12N 2760/18122 (2013.01); C12N 2760/18134 (2013.01); C12N 2760/18171 (2013.01); C12N 2760/18522 (2013.01); C12N 2760/18534 (2013.01); C12N 2760/18571 (2013.01)]

5 Claims

1. A method for immunizing a mammalian subject, comprising,

a) providing

i) a first RSV experienced mammalian subject containing Respiratory Syncytial Virus (RSV) neutralizing antibodies, and

ii) a first composition comprising recombinant chimeric Newcastle Disease virus-like particles (ND VLPs) that contain

1) stabilized pre-fusion RSV F protein ectodomain comprising SEQ ID NO:06, and

2) RSV G ectodomain protein, and

b) administering an immunologically effective amount of the first composition to the first RSV experienced mammalian subject to produce a first immunized mammalian subject, wherein said administering is under conditions that increase the level of the RSV neutralizing antibodies in said first immunized mammalian subject.