| CPC A61B 5/0275 (2013.01) [A61B 5/0071 (2013.01); A61B 5/0261 (2013.01); A61B 5/489 (2013.01)] | 22 Claims |

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1. A computer implemented method for detecting one or more areas having an abnormal perfusion pattern in tissue of a subject during a medical procedure, the method comprising:
continuously acquiring fluorescence images of the tissue, wherein the fluorescence images are associated with a fluorescent output signal correlated with an input signal defined by a series of boluses of at least one fluorescent imaging agent, and wherein the series of boluses is administered with a predefined and controlled duration between subsequent boluses,
analyzing the fluorescence images,
obtaining, defining and/or determining a normal perfusion pattern, at least in an intensity domain and/or in a time domain, and
obtaining at least a first body kernel which is the filter imposed by the subjects body on said at least one fluorescent imaging agent in a tissue area with a normal perfusion pattern, and/or
obtaining at least a second body kernel which is the filter imposed by the subjects body on said at least one fluorescent imaging agent in a tissue area with an abnormal perfusion pattern,
such that the at least first body kernel and/or the at least second body kernel becomes at least one transfer function between the input signal and the fluorescent output signal,
detecting, in the fluorescence images, possible tissue areas with an abnormal perfusion pattern based on the at least first body kernel and/or the at least second body kernel.
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